Scientist, Potency and Functional Characterization
Bristol-Myers Squibb
Scientist, Potency and Functional Characterization
Job Description
Generate monoclonal cell lines and establish cell banks by transducing cells with viral vectors, expanding the cultures, isolating monoclonal populations through cell sorting, banking the validated cell lines, and performing functional qualification using lot-release potency and flow cytometry assays. Design and execute laboratory experiments to optimize DNA/RNA transfection, viral transduction, flow cytometry and plate based potency reporter gene assay parameters and procedures to meet the pre-defined analytical target profile. Contribute to method qualification and transfer activities based on ICH/USP guidelines. Analyze complex data sets, perform data trending and visualization, and maintain detailed experimental records in ELN for reproducibility and quality assurance. Document cell line generation processes and experimental findings, contributing to SOPs, technical reports, and regulatory filings to ensure compliance and support innovation. Cultivate and maintain cell lines and primary cells essential for innovative assay development. Manage lab operations by overseeing inventories, scheduling equipment maintenance, and implementing process improvements for efficiency and productivity. Work-life programs include paid national holidays and optional holidays, Global Shutdown Days between Christmas and New Year's holiday, up to 120 hours of paid vacation, up to two (2) paid days to volunteer, sick time off, and summer hours flexibility. Ph.D. in Immunology, Cell Biology, Bioengineering, or a related field with 0-3 years of industry experience; or an MS/BS with 5+ years of relevant laboratory experience. Proficiency in cell culture, viral vector transduction, cell line engineering, flow cytometry, and relevant analytical assays (e.g., ELISA). Experience with JMP and SoftmaxPro softwares is preferred. Experience with vector construct design is highly desirable. Background in DNA/RNA transfection, viral vector transduction, and potency release method development is preferred. Strong organizational, documentation, and communication skills. Ability to thrive in a collaborative, fast-paced, and dynamic environment.
Key Responsibilities
- Generate monoclonal cell lines and establish cell banks by transducing cells with viral vectors, expanding the cultures, isolating monoclonal populations through cell sorting, banking the validated cell lines, and performing functional qualification using lot-release potency and flow cytometry assays
- Design and execute laboratory experiments to optimize DNA/RNA transfection, viral transduction, flow cytometry and plate based potency reporter gene assay parameters and procedures to meet the pre-defined analytical target profile
- Contribute to method qualification and transfer activities based on ICH/USP guidelines
- Analyze complex data sets, perform data trending and visualization, and maintain detailed experimental records in ELN for reproducibility and quality assurance
- Document cell line generation processes and experimental findings, contributing to SOPs, technical reports, and regulatory filings to ensure compliance and support innovation
- Cultivate and maintain cell lines and primary cells essential for innovative assay development
- Manage lab operations by overseeing inventories, scheduling equipment maintenance, and implementing process improvements for efficiency and productivity
Required Qualifications
- Ph.D. in Immunology, Cell Biology, Bioengineering, or a related field with 0-3 years of industry experience
- MS/BS with 5+ years of relevant laboratory experience
Preferred Qualifications
- Experience with JMP and SoftmaxPro softwares
- Experience with vector construct design
- Background in DNA/RNA transfection, viral vector transduction, and potency release method development
Skills Required
- Proficiency in cell culture, viral vector transduction, cell line engineering, flow cytometry, and relevant analytical assays (e.g., ELISA)
- Strong organizational, documentation, and communication skills
- Ability to thrive in a collaborative, fast-paced, and dynamic environment
Benefits & Perks
- Paid national holidays and optional holidays
- Global Shutdown Days between Christmas and New Year's holiday
- Up to 120 hours of paid vacation
- Up to two (2) paid days to volunteer
- Sick time off
- Summer hours flexibility
Locations
- Seattle 400 Dexter WA, United States
Salary
Estimated Salary Rangemedium confidence
100,000 - 150,000 USD / yearly
Source: ai estimated
* This is an estimated range based on market data and may vary based on experience and qualifications.
Skills Required
- Proficiency in cell culture, viral vector transduction, cell line engineering, flow cytometry, and relevant analytical assays (e.g., ELISA)intermediate
- Strong organizational, documentation, and communication skillsintermediate
- Ability to thrive in a collaborative, fast-paced, and dynamic environmentintermediate
Required Qualifications
- Ph.D. in Immunology, Cell Biology, Bioengineering, or a related field with 0-3 years of industry experience (experience)
- MS/BS with 5+ years of relevant laboratory experience (experience)
Preferred Qualifications
- Experience with JMP and SoftmaxPro softwares (experience)
- Experience with vector construct design (experience)
- Background in DNA/RNA transfection, viral vector transduction, and potency release method development (experience)
Responsibilities
- Generate monoclonal cell lines and establish cell banks by transducing cells with viral vectors, expanding the cultures, isolating monoclonal populations through cell sorting, banking the validated cell lines, and performing functional qualification using lot-release potency and flow cytometry assays
- Design and execute laboratory experiments to optimize DNA/RNA transfection, viral transduction, flow cytometry and plate based potency reporter gene assay parameters and procedures to meet the pre-defined analytical target profile
- Contribute to method qualification and transfer activities based on ICH/USP guidelines
- Analyze complex data sets, perform data trending and visualization, and maintain detailed experimental records in ELN for reproducibility and quality assurance
- Document cell line generation processes and experimental findings, contributing to SOPs, technical reports, and regulatory filings to ensure compliance and support innovation
- Cultivate and maintain cell lines and primary cells essential for innovative assay development
- Manage lab operations by overseeing inventories, scheduling equipment maintenance, and implementing process improvements for efficiency and productivity
Benefits
- general: Paid national holidays and optional holidays
- general: Global Shutdown Days between Christmas and New Year's holiday
- general: Up to 120 hours of paid vacation
- general: Up to two (2) paid days to volunteer
- general: Sick time off
- general: Summer hours flexibility
Tags & Categories
Scientist, Potency and Functional Characterization
Bristol-Myers Squibb
Scientist, Potency and Functional Characterization
Job Description
Generate monoclonal cell lines and establish cell banks by transducing cells with viral vectors, expanding the cultures, isolating monoclonal populations through cell sorting, banking the validated cell lines, and performing functional qualification using lot-release potency and flow cytometry assays. Design and execute laboratory experiments to optimize DNA/RNA transfection, viral transduction, flow cytometry and plate based potency reporter gene assay parameters and procedures to meet the pre-defined analytical target profile. Contribute to method qualification and transfer activities based on ICH/USP guidelines. Analyze complex data sets, perform data trending and visualization, and maintain detailed experimental records in ELN for reproducibility and quality assurance. Document cell line generation processes and experimental findings, contributing to SOPs, technical reports, and regulatory filings to ensure compliance and support innovation. Cultivate and maintain cell lines and primary cells essential for innovative assay development. Manage lab operations by overseeing inventories, scheduling equipment maintenance, and implementing process improvements for efficiency and productivity. Work-life programs include paid national holidays and optional holidays, Global Shutdown Days between Christmas and New Year's holiday, up to 120 hours of paid vacation, up to two (2) paid days to volunteer, sick time off, and summer hours flexibility. Ph.D. in Immunology, Cell Biology, Bioengineering, or a related field with 0-3 years of industry experience; or an MS/BS with 5+ years of relevant laboratory experience. Proficiency in cell culture, viral vector transduction, cell line engineering, flow cytometry, and relevant analytical assays (e.g., ELISA). Experience with JMP and SoftmaxPro softwares is preferred. Experience with vector construct design is highly desirable. Background in DNA/RNA transfection, viral vector transduction, and potency release method development is preferred. Strong organizational, documentation, and communication skills. Ability to thrive in a collaborative, fast-paced, and dynamic environment.
Key Responsibilities
- Generate monoclonal cell lines and establish cell banks by transducing cells with viral vectors, expanding the cultures, isolating monoclonal populations through cell sorting, banking the validated cell lines, and performing functional qualification using lot-release potency and flow cytometry assays
- Design and execute laboratory experiments to optimize DNA/RNA transfection, viral transduction, flow cytometry and plate based potency reporter gene assay parameters and procedures to meet the pre-defined analytical target profile
- Contribute to method qualification and transfer activities based on ICH/USP guidelines
- Analyze complex data sets, perform data trending and visualization, and maintain detailed experimental records in ELN for reproducibility and quality assurance
- Document cell line generation processes and experimental findings, contributing to SOPs, technical reports, and regulatory filings to ensure compliance and support innovation
- Cultivate and maintain cell lines and primary cells essential for innovative assay development
- Manage lab operations by overseeing inventories, scheduling equipment maintenance, and implementing process improvements for efficiency and productivity
Required Qualifications
- Ph.D. in Immunology, Cell Biology, Bioengineering, or a related field with 0-3 years of industry experience
- MS/BS with 5+ years of relevant laboratory experience
Preferred Qualifications
- Experience with JMP and SoftmaxPro softwares
- Experience with vector construct design
- Background in DNA/RNA transfection, viral vector transduction, and potency release method development
Skills Required
- Proficiency in cell culture, viral vector transduction, cell line engineering, flow cytometry, and relevant analytical assays (e.g., ELISA)
- Strong organizational, documentation, and communication skills
- Ability to thrive in a collaborative, fast-paced, and dynamic environment
Benefits & Perks
- Paid national holidays and optional holidays
- Global Shutdown Days between Christmas and New Year's holiday
- Up to 120 hours of paid vacation
- Up to two (2) paid days to volunteer
- Sick time off
- Summer hours flexibility
Locations
- Seattle 400 Dexter WA, United States
Salary
Estimated Salary Rangemedium confidence
100,000 - 150,000 USD / yearly
Source: ai estimated
* This is an estimated range based on market data and may vary based on experience and qualifications.
Skills Required
- Proficiency in cell culture, viral vector transduction, cell line engineering, flow cytometry, and relevant analytical assays (e.g., ELISA)intermediate
- Strong organizational, documentation, and communication skillsintermediate
- Ability to thrive in a collaborative, fast-paced, and dynamic environmentintermediate
Required Qualifications
- Ph.D. in Immunology, Cell Biology, Bioengineering, or a related field with 0-3 years of industry experience (experience)
- MS/BS with 5+ years of relevant laboratory experience (experience)
Preferred Qualifications
- Experience with JMP and SoftmaxPro softwares (experience)
- Experience with vector construct design (experience)
- Background in DNA/RNA transfection, viral vector transduction, and potency release method development (experience)
Responsibilities
- Generate monoclonal cell lines and establish cell banks by transducing cells with viral vectors, expanding the cultures, isolating monoclonal populations through cell sorting, banking the validated cell lines, and performing functional qualification using lot-release potency and flow cytometry assays
- Design and execute laboratory experiments to optimize DNA/RNA transfection, viral transduction, flow cytometry and plate based potency reporter gene assay parameters and procedures to meet the pre-defined analytical target profile
- Contribute to method qualification and transfer activities based on ICH/USP guidelines
- Analyze complex data sets, perform data trending and visualization, and maintain detailed experimental records in ELN for reproducibility and quality assurance
- Document cell line generation processes and experimental findings, contributing to SOPs, technical reports, and regulatory filings to ensure compliance and support innovation
- Cultivate and maintain cell lines and primary cells essential for innovative assay development
- Manage lab operations by overseeing inventories, scheduling equipment maintenance, and implementing process improvements for efficiency and productivity
Benefits
- general: Paid national holidays and optional holidays
- general: Global Shutdown Days between Christmas and New Year's holiday
- general: Up to 120 hours of paid vacation
- general: Up to two (2) paid days to volunteer
- general: Sick time off
- general: Summer hours flexibility
Tags & Categories